Laboratory of Genetics and Epigenetics of Human Diseases

The LGEHD laboratory was established in July 2020. The major topic of the Laboratory research is genetic and epigenetic control on immune response regulating molecules in disease development, progression and treatment (with special focus on the pathogenic role of immune checkpoints molecules in human diseases). In particular, our studies concentrate on:

  • investigation of inherited variations within genes regulating the immune response in relation to the risk of developing cancer, autoimmune and other diseases;
  • evaluation of the relationship between epigenetic modifications (e.g. DNA methylation and microRNA) and the risk of disease development and progression;
  • searching for biomarkers of response to immunotherapeutic treatment;
  • examination of the functional relevance of inherited genetic variants.

Currently, 2 research grants are being carried out in the Laboratory:

  • Grant OPUS 17 financed by the National Science Centre: “The immune checkpoints in non-small cell lung cancer-research on genetic and epigenetic mechanism regulating mRNA and protein expression” principal investigator: Monika Jasek MSc, PhD, DSc; 2 257 528 PLN (2020-2023) (grant No. 2019/33/B/NZ5/03029)
  • Project financed by the Hr. Potocki Foundation: “The influence of genetic variations in genes coding PD-1/PD-L1 on the risk of kidney cancer and the course of disease” principal investigator: Lidia Karabon MSc, PhD, DSc 95 000 PLN (2020-2021)
Laboratory Head

Head: Lidia Karabon MSc, PhD, DSc (Associate Professor, 0000-0001-8172-0971)

Education: XIV High School in Wroclaw, Wroclaw University of Science and Technology, Faculty of Chemistry; PhD degree 2003 and habilitation 2012 in IIET PAS;

Employment: 1988-1991 Wroclaw University of Science and Technology, Faculty of Chemistry; 1991-2001 K. Dluski Hospital, Bone Marrow Transplantation Unit; 2002-2005 Lower Silesian Centre for Cellular Transplantation, head of the HLA Laboratory; since 2006, IIET PAS;

International internships: 2001-Medical University, Vienna, Austria-1 month; LifeCodes Company Stamford, USA-1 week; 2004-University of Pennsylvania School of Medicine, Philadelphia, USA-1 year; 2016-Institute of Gene Biology, RAS Russia-3 months; 2018-University of Thessaly, Greece-1 week. Author of 54 publications from the JCR list, including: PlosOne, Journal of Immunology Research, Cancers (Basel), Frontiers of Genetics, Leukemia, Bone Marrow Transplantation, British Journal of Hematology, Immunology, Immunology Letters, Immunogenetics, Nucleic Acid Research, Journal of Neuroimmunology, Urological Oncology;

Membership in Societies: European Federation for Immunogenetics; Polish Society for Immunogenetics (President since 2014); Polish Society of Fundamental and Clinical Immunology; married, 4 children, hobbies: movies, trips, ceramics

Team
Associate Professor:

Technical Staff:

PhD Students:

Research methods
  • molecular biology
  • genotyping of single nucleotide polymorphisms (RFLP, HRM, TaqMan Probes)
  • methylation study (MS-HRM)
  • gene expression (RT-qPCR)
  • microRNA expression (RT-qPCR)
  • gene (siRNA, As-OND) and microRNA silencing
  • promoter activity (luciferase assay)
  • tissue culture
  • culture of cell line and mononuclear cell from human blood
  • cell transfection (lipotransfection, nucleofection)
  • ELISA, Western- blot

Most important projects (during the last 10 years)
  • the National Science Centre Grant OPUS 17: ” The immune checkpoints in non-small cell lung cancer-research on genetic and epigenetic mechanism regulating mRNA and protein expression” principal investigator: Monika Jasek MSc, PhD, DSc; 2 257 528 PLN (2020-2023) (grant No. 2019/33/B/NZ5/03029)
  • the project financed by the Hr. Potocki Foundation “The influence of genetic variations in genes coding PD-1/PD-L1 on the risk of kidney cancer and the course of disease” principal investigator: Lidia Karabon MSc, PhD, DSc; 95 000 PLN (2020-2021)
Selected publications
  • Jasek M, Bojarska-Junak A, Sobczyński M, Wagner M, Chocholska S, Roliński J, Wołowiec D and Karabon L, “Association of Common Variants of TNFSF13 and TNFRSF13B Genes with CLL Risk and Clinical Picture, as well as Expression of their Products-APRIL and TACI Molecules” Cancers (Basel). 2020 Oct 6;12(10):E2873. doi:10.3390/cancers12102873. PMID: 33036273